| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
ALEXA FLUOR® 594 |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
HOXA5 |
| Specificity: |
This antibody reacts specifically with HOXA5 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
N/A |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human HOXA5 |
| Gene ID: |
3202 |
| Swiss Prot: |
N/A |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus) |
| Cross Reactive Species details: |
No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species. |
| Background information: |
HOXA5 upregulates both p53 promoter-reporter constructs and endogenous p53 synthesis, leading to apoptosis. HOXA5 is detectable in approximately one-third of primary tumors. Lack of HOXA5 expression strongly correlates with methylation of its promoter region, suggesting a causal role for methylation in the silencing of HOXA5 gene expression. HOXA5 expression is an important step in tumorigenesis and loss of expression of p53 in human breast cancer may be primarily due to lack of expression of HOXA5. |
| Purification method: |
Purified by Protein A. |
| Storage: |
Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months. |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
Homeo box 1C antibody Homeobox A5 antibody Homeobox protein HOXA5 antibody HOX 1 antibody HOX 1.3 antibody HOX 1C antibody HOX1 antibody HOX1.3 antibody HOX1C antibody; HXA5_HUMAN. |
| Also known as: |
HOXA5 Polyclonal Antibody |
| Other name: |
Anti-HOXA5 Polyclonal |
| Advisory: |
Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom. |
| Properties: |
For facs or microscopy Alexa 1 conjugate. |
| Conjugation: |
Alexa Fluor,ALEXA FLUOR® 594 |
| Conjugated: |
Alexa conjugate 1 |
| Description: |
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. |
| Group: |
Polyclonals and antibodies |
| About: |
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. |