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redcoon België GENTAUR BVBA

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1040 Brussels BELGIUM

Tel + 32 16 58 90 45 

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Matched-sets of Human and Mouse iPS Cell Lines

Jump start your iPS cell research with pre-made, high quality iPS cell lines
• iPS cell lines made using Oct4, Sox2, c-Myc, and KLF4
• Study the induced pluripotency process
• Measure mRNA and microRNA signatures
• Identify novel epigenetic markers to study pattern formation
• Develop directed differentiation protocols
 

• Human certified iPS Cell Line catalog# SC101A-1
• Mouse certified iPS Cell Line catalog# SC201A-1

 

All iPS Cell lines are certified to be pluripotent through iPS Cell Marker staining

Stem cell markers for SSEA4/1 and Nanog were determined by immunocytochemistry using primary antibodies for SSEA1 (Millipore), SSEA4 (Abcam) and Nanog (Abcam) followed by fluorescent-labeled secondary Alexa594 antibodies (Invitrogen). Detection of Alkaline Phosphatase activity was performed using the AP Detection kit (Millipore). Teratoma assays were performed by subcutaneous injection of 1x10^6 iPS cells into the dorsal flank of SCID mice. Teratomas were visualized after 4 weeks with hematoxylin and eosin staining.

 

How the iPS Cell Lines are made

Human foreskin fibroblasts (HFFs) or Mouse embryonic fibroblasts (MEFs) are transduced with high-titer iPSC "clean" virus to induce pluripotency. The result is high quality iPS cells with no fluorescent markers or drug selection required. The entire process takes up to 6 weeks and these iPS cell lines are pre-made and validated - ready for your research.

 

iPS Cell Line Differentiation Studies

Mouse iPS Cells are capable of producing multiple neural lineages

• Develop directed differentiation protocols
• Make new transgenic lines
• Confirm lineage specification using SBI's Differentiation Lenti-reporters
 

Quality Source and Feeder Cells for Reliable Reprogramming

Human foreskin fibroblasts, neonatal (Cat#PC501A-HFF)
• Highly pure neonatal foreskin fibroblasts optimized for iPSC generation
• Isolated from individual foreskin, single genetic background
• Measure mRNA and microRNA signatures
• Two times more cells/vial compared with competitors’ product


 

Highly pure neonatal foreskin fibroblasts for Feeder Cells (Cat#PC502B-HFF)
• Highly pure neonatal foreskin fibroblasts
• Pooled neonatal foreskin fibroblasts optimized as feeder cells
 


 

Human Foreskin Fibroblasts (Primary cells, Passage 1)

 

Human and Mouse iPS Cell Line Sets

Human iPS Cell Line Set [HFF and iPS cells] 2x10^5 cells each SC101A-1
Mouse iPS Cell Line Set [MEF and iPS cells] 2x10^5 cells each SC201A-1
 

The pre-made iPS Cell lines are available as matched sets of the source fibroblast cells along with their induced pluripotent cells. The iPS Cell Lines come with 50 ml of SBI's StemPure™ complete media.
 

Related iPSC Products: Source Cells, Feeder Cells and Stem Cell Media

Human Foreskin Fibroblasts for iPSC Construction (Passage P1, from individual genetic source) 1x10^6 cells PC501A-HFF
Human Foreskin Fibroblasts as Feeder Cells (Passage P1, pooled samples) 1x10^6 cells PC502B-HFF
StemPure™ ESC/iPSC Growth Medium (500ml) 500 ml SC050M-1
 
Highly pure Neonatal foreskin fibroblasts optimized for iPSC genereation (cat# PC501A-HFF)
Pooled Neonatal foreskin fibroblasts optimized for use as feeder cells (cat# PC502B-HFF)
Grow your embryonic and iPS cells reliably in SBI's StemPure™ media (cat# SC050M-1)
 

 

 

Copyright © 2009 GENTAUR Last modified: 07/23/10